利用亲水无规共聚物制备银纳米颗粒及抗菌应用研究

doi:10.19491/j.issn.1001-9278.2020.11.003

中国塑料 ›› 2020, Vol. 34 ›› Issue (11): 12-18.DOI: 10.19491/j.issn.1001-9278.2020.11.003

利用亲水无规共聚物制备银纳米颗粒及抗菌应用研究

杨禄¹^,², 陈韩婷¹

^1.江南大学化学与材料工程学院，江苏无锡 214122
^2.无锡麦克罗新材料科技有限公司，江苏无锡 214122

收稿日期:2020-04-01 出版日期:2020-11-26 发布日期:2020-11-20

Preparation of Silver Nanoparticle via Hydrophilic Random Copolymers and Its Antibacterial Application

YANG Lu¹^,², CHEN Hanting¹

^1.School of Chemical and Material Engineering，Jiangnan University，Wuxi 214122，China
^2.Wuxi MACRO NMS Co，Ltd，Wuxi 214122，China

Received:2020-04-01 Online:2020-11-26 Published:2020-11-20
Contact: YANG Lu E-mail:13861747479@126.com

摘要/Abstract

摘要：

以纯亲水无规共聚物poly（DMAEMA-co-AA）的水相自组装体为模板，研究了其微观纳米自组装体的形貌结构特点，通过原位还原法制备银纳米颗粒，并研究其在抗菌包装中的应用。结果表明，低pH值时，纯亲水无规共聚物poly（DMAEMA-co-AA）形成微孔棒状及棒状的二级自组装体（球状或立方体状）的稳定结构；随着银还原率的提高，银纳米颗粒从球状或立方体状解体为小球状；抗菌薄膜对数抗菌值在4.5以上，用于抗菌包装后常温食品存储时间延长至4倍以上。

关键词: 亲水无规共聚物, 水相自组装, 抗菌材料, 银纳米颗粒

Abstract:

A self?assembled system was prepared using pure hydrophilic random copolymer poly（DMAEMA-co-AA） as a template， and its morphology and structure were investigated. Silver nanoparticles were then prepared by an in-situ reduction method， and their application in antibacterial packaging was studied. The results indicated that the pure hydrophilic random copolymer poly（DMAEMA-co-AA） could form the stable structures of microporous rod-like and rod?like secondary self-assembled systems in spherical or cubic shape at low pH value. With the increase of silver reduction rate， the silver nanoparticles disintegrated from the spherical or cubic shape to a small spherical shape. The logarithm antibacterial value of the antibacterial film was more than 4.5. The storage time of fruits at room temperature increased by more than 4 times after the silver nanoparticles were used for antibacterial packaging.

Key words: hydrophilic random copolymer, aqueous self?assembly, antibacterial material, silver nanoparticle

中图分类号:

TQ325.1⁺4

杨禄, 陈韩婷. 利用亲水无规共聚物制备银纳米颗粒及抗菌应用研究[J]. 中国塑料, 2020, 34(11): 12-18.

YANG Lu, CHEN Hanting. Preparation of Silver Nanoparticle via Hydrophilic Random Copolymers and Its Antibacterial Application[J]. China Plastics, 2020, 34(11): 12-18.

图/表 8

DMAEMA∶AA (mol/mol)	AA含量/%	M_n/10⁴	M_w/10⁴	多分散系数
30∶70	51.6	2.37	2.79	1.18
70∶30	16.6	1.83	2.11	1.15

表1 poly(DMAEMA?co?AA)的相对分子质量及DMAEMA与AA实际组成比

Tab.1 The mole ratios of monomers and GPC results of the poly(DMAEMA?co?AA) family

图1 引发剂含量对poly(DMAEMA?co?AA)聚合反应的影响

Fig.1 The effect of different initiator dosage on the polymerization of poly(DMAEMA?co?AA)

图2 反应温度对poly(DMAEMA?co?AA)聚合反应的影响

Fig.2 The effect of different reaction temperature on the polymerization of poly(DMAEMA?co?AA)

图3 poly(DMAEMA?co?AA)的ATR?FTIR曲线

Fig.3 ATR?FTIR curves of poly(DMAEMA?co?AA)

图4 poly(DMAEMA?co?AA)自组装体的TEM及SEM照片

Fig.4 TEM and SEM of poly(DMAEMA?co?AA) self?assemblies

图5 pH=2.0时AgNPS的TEM照片

Fig.5 TEM of AgNPS at pH=2.0

序号	测试菌种	菌种浓度/×10⁷ cfu·mL^-1	回收菌种浓度/cfu?cm^-2			对数抗菌值R
序号	测试菌种	菌种浓度/×10⁷ cfu·mL^-1	样品	接触时间为0 h	接触时间为24 h	对数抗菌值R
1	大肠杆菌ATCC 8739	2.6	F1	—	9.2×10²	4.513 3
	大肠杆菌ATCC 8739	2.6	对照样品	2.3×10⁶	3.0×10⁷	—
	金黄色葡萄球菌 ATCC 6538P	2.3	F1	—	6.3×10²	4.562 4
	金黄色葡萄球菌 ATCC 6538P	2.3	对照样品	2.3×10⁶	2.3×10⁷	—
2	大肠杆菌ATCC 8739	2.4	F2	—	5.7×10²	4.706 5
	大肠杆菌ATCC 8739	2.4	对照样品	2.5×10⁶	2.9×10⁷	—
	金黄色葡萄球菌 ATCC 6538P	2.3	F2	—	4.4×10²	4.678 8
	金黄色葡萄球菌 ATCC 6538P	2.3	对照样品	2.1×10⁶	2.1×10⁷	—
3	大肠杆菌ATCC 8739	2.5	F3	—	4.9×10	5.671 5
	大肠杆菌ATCC 8739	2.5	对照样品	2.8×10⁶	2.3×10⁷	—
	金黄色葡萄球菌 ATCC 6538P	2.5	F3	—	8.3×10	5.495 9
	金黄色葡萄球菌 ATCC 6538P	2.5	对照样品	2.6×10⁶	2.6×10⁷	—

表2 涂膜后PE薄膜的抗菌性

Tab.2 Antimicrobial activity of PE film

图6 常温保鲜测试对比照片

Fig.6 The photo images of preserving freshness test at normal atmospheric temperature

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[1]	杨禄, 陈韩婷. 纯亲水无规共聚物pH响应自组装及活性物控释[J]. 中国塑料, 2020, 34(9): 9-15.
[2]	杨禄, 陈韩婷. 纯亲水自组装体构建抗菌纳米材料[J]. 中国塑料, 2020, 34(10): 12-17.
[3]	李毕忠李泽国阳文崔辉仙马波路明杰. 食品活性包装用抗菌材料技术进展[J]. 中国塑料, 2012, 26(03): 10-16 .
[4]	王广莉;汤瑜. 抗菌纳米SiOx粉体应用于PVC试验研究[J]. 中国塑料, 2009, 23(08): 79-82 .

利用亲水无规共聚物制备银纳米颗粒及抗菌应用研究

Preparation of Silver Nanoparticle via Hydrophilic Random Copolymers and Its Antibacterial Application

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