Abstract: Poly(ε-caprolactone) (PCL) films were degraded by lipases and then were characterized by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), X-ray diffraction (XRD), nuclear magnetic resonance (NMR) and mass spectrometry (MS).The results indicated that the PCL films achieved a weight loss rate of 86.9 wt % when digested with 30 U/mL of lipase at 45 ℃ for 20 h.SEM observation showed that there were lots of pores formed on the film surface with an increase of degradation time, and the size of pores tended to increase with degradation time.DSC and XRD analyses indicated that the relative crystallinity of PCL gradually decreased with an increase of degradation time.The thermal stability of PCL films was found to decrease with an increase of degradation time according to the TGA results.NMR analysis demonstrated that there was no change for PCL films in chemical composition before and after enzymatic degradation.