Abstract: A type of antibacterial films was prepared by a casting method using TiO2 nanoparticles (Nano-TiO2) as an antibacterial agents and low-density polyethylene (PE-LD) resin as a matrix, followed by hot compression with polyethylene terephthalate (PET) film as an outer layer. The mechanical, antibacterial and optical properties of the composite films were evaluated. The results indicated that, as the proportion of increases, the mechanical and antibacterial properties of the films were enhanced with increasing the loading of Nano-TiO2, whereas the optical properties tended to decrease. When 2.5 wt % of Nano-TiO2 was added, the tensile and haze strength of the films was higher than the pristine one. However, their light transmittance became smaller, but their gas resistance, water resistance and antibacterial effect became better. The composite films developed by this work can be used for the antimicrobial packaging of food.