Abstract: To understand the preparation process and skin-puncturing effect of microneedles with stratified solubility for efficient use of lidocaine, the polymer microneedles were prepared by two casting methods using polydimethylsiloxane as a negative model and the polyvinyl alcohol/sodium hyaluronate/polyvinylpyrrolidone compound as a composite matrix. The morphology of the resulting polymer microneedles was observed by a microscope, and their height was measured. The puncturing test was conducted on the abdominal skin of a mouse, and then the puncturing effect of microneedles was investigated upon the obtained frozen sections. The experimental results indicated that the lidocaine-containing polymer microneedles achieved a formation rate of 100 %, and lidocaine was mainly distributed in the needle bodies of the microneedles at a height of 450 μm. The microneedles were able to penetrate the skin just after reaching the dermis layer by giving a channel with a depth of about 250 μm through the skin. In addition, 90 wt% of the needle bodies dissolved rapidly within 5 min, and the molding cycle was shortened within 4 hours.