Preparation of cellulose acetate porous membrane and its adsorption for protein

doi:10.19491/j.issn.1001-9278.2025.03.007

Abstract

Abstract:

In this study， cellulose acetate porous membranes with an adjustable structure and a high water flux were prepared by using cellulose acetate as a raw material， acetone as a solvent， and water as a non⁃solvent. Carboxyl groups were introduced for selective separation of protein through the oxidation of tetramethylpyridine oxide/sodium bromide/sodium hypochlorite. With a porosity of 92.5 % and a water flux of 1 655.7 L/（m²·h）， the cellulose acetate porous membranes exhibited optimal comprehensive performance at a cellulose acetate concentration of 10 wt%， a coagulation bath temperature of 10 ºC， and a non⁃solvent water content of 30 wt%. After oxidation， the cellulose acetate porous membrane obtained an adsorption rate of 2 806.1 mg/g for lysozyme at pH 6.

Key words: cellulose acetate, non solvent induced phase separation, porous membrane

CLC Number:

TQ323.72⁺1

HOU Wenjie, YANG Na, REN Jianfei, GAO Dahai, GUO Gaiping, DAI Yuhua. Preparation of cellulose acetate porous membrane and its adsorption for protein[J]. China Plastics, 2025, 39(3): 36-40.

Figures/Tables 8

Fig.1. Preparation process diagram of cellulose acetate porous membrane

Fig.2. Morphological images of cellulose acetate porous membranes with different concentrations（DC⁃a is a sample with a concentration of 10 % cellulose acetate

样品	浓度/%	孔隙率/%	水通量/L $∙$ m^-2 $∙$ h^-1
DC⁃a	10	91.1	650.6
DC⁃b	11	89.4	188.1
DC⁃c	12	89.1	141.8
DC⁃d	13	88.9	79.0
DC⁃e	14	87.7	25.3
DC⁃f	15	86.4	25.1

样品	浓度/%	孔隙率/%	水通量/L $∙$ m^-2 $∙$ h^-1
DC⁃a	10	91.1	650.6
DC⁃b	11	89.4	188.1
DC⁃c	12	89.1	141.8
DC⁃d	13	88.9	79.0
DC⁃e	14	87.7	25.3
DC⁃f	15	86.4	25.1

Tab.1. Porosity and water flux of different concentrations of cellulose acetate

样品	温度/℃	孔隙率/%	水通量/L·m^-2·h^-1
CTA⁃a	0	91.0	622.6
CTA⁃b	5	92.1	809.7
CTA⁃c	10	92.5	1 655.7
CTA⁃d	15	92.3	1 449.2
CTA⁃e	20	92.1	823.0

Tab.2. Porosity and water flux of different concentrations of cellulose acetate

Fig.3. Morphological images of cellulose acetate porous membranes at different coagulation bath temperatures

样品	水含量/%	孔隙率/%	水通量/L·m^-2·h^-1
WA⁃a	20	90.2	1 799.8
WA⁃b	25	90.2	1 209.8
WA⁃c	30	91.1	650.6
WA⁃d	35	92.2	1 110.9
WA⁃e	40	93.6	38 038.6

Tab.3. Porosity and water flux of cellulose acetate/acetone solution with different water additions

Fig.4. Morphological images of cellulose acetate/acetone solution with different amounts of water

Fig.5. Hydrolysis infrared spectrum of cellulose acetate porous membrane

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