Preparation and performance analysis of porous scaffold materials with sustained release activity

doi:10.19491/j.issn.1001-9278.2022.11.011

Abstract

Abstract:

Polycaprolactone （PCL）/curcumin （CUR） porous scaffold materials were prepared using a solution⁃casting method， and their porosity and drug loading and sustained release behaviors were investigated using scanning electron microscopy and thermogravimetric analysis. The in vitro drug release model of the scaffold materials was also analyzed. The results indicated that the drug release model of the scaffold materials conformed to the first⁃order equation at a CUR content of 2 wt%， a PCL concentration of 10 wt% in acetic acid， and a chitosan content of 2.86 wt% in the scaffold materials. The scaffold materials presented porosity of over 95 %， resulting in a drug loading of 1.63 %. In the phosphate buffer solution， the cumulative release rate of CUR for 90 h was 76.2 %. For other formulations， the scaffold materials showed porosity of above 95 %， and their cumulative slow⁃release rate of CUR for 90 h was between 60 % and 87 %. This demonstrated that the developed scaffold materials had ideal porosity and obvious sustained⁃release effect of CUR drug， exhibiting a good application prospect in the tissue⁃engineering field.

Key words: curcumin, polycaprolactone, scaffold, sustained release, drug release model

CLC Number:

TQ323.4

WANG Pei, NIU Lili, LI Jingyu, GENG Hongmei. Preparation and performance analysis of porous scaffold materials with sustained release activity[J]. China Plastics, 2022, 36(11): 73-78.

Figures/Tables 12

Fig.1. SEM images of PCL⁃CUR scaffold

Fig.2. DTG curves of the samples

PCL浓度/%	CUR含量/%	壳聚糖含量/%	孔隙率/%
10	2	0	19.09
10	2	1.43	94.67
10	2	2.86	95.26
10	2	5.71	98.07
10	2	8.57	97.91
10	1	2.86	95.33
8	2	2.86	96.42

Tab.1. Porosities of the scaffold materials

Fig.3. Effect of CUR content on drug loading capacity of the scaffold

Fig.4. Effect of chitosan content on drug loading capacity of the scaffold

Fig.5. Effect of CUR content on cumulative drug release rate

Fig.6. Relationship between cumulative drug release rate and chitosan content

Fig.7. Relationship between cumulative drug release rate and PCL concentration

拟合模型	拟合方程^a
零级模型	M_t /M_∞=kt
1级模型	ln（1-M_t /M_∞）=-kt
Higuchi模型	M_t/M_∞=kt^1/2

Tab.2. In vitro drug release model

Fig.8. Zero⁃order model fitting results

Fig.9. Higuchi model fitting results

Fig.10. One⁃order model fitting results

References 15

1	Ishiguro H， Kaito T， Yarimitsu S， et al. Intervertebral discregeneration with an adipose mesenchymal stem cell⁃derived tissue⁃engineered construct in a rat nucleotomy model［J］. Acta Biomater， 2019，87：118⁃129.
2	Song J Y， Ryu H， Lee J M， et al. Conformal fabrication of an electrospun nanofiber mat on a 3D ear cartilage⁃shaped hydrogel collector based on hydrogel⁃assisted electrospinning［J］. Nanoscale Research Letters， 2021，16（1）：116⁃127.
3	王月波，汪焰恩，魏生民，等.人工骨支架结构设计与实现［J］.西安工业大学学报，2012，32（4）：282⁃283.
	ＷANG Y B，ＷANG Ｙ E，ＷEI Ｓ M，et al. Research implementation of artificial bone scaffold structure design［J］. Journal of Northwestern Polytechnical University， 2012，32（4）：282⁃283.
4	徐尚龙. 基于细胞沉积的人工骨微管设计及三维流场分析［J］.机械工程学报，2005，41（4）：154⁃156.
	XU S L. Bionic design and 3D flow analysis of artificial bone microtubules and fabrication based on cell attachment［J］.Chinese Journal of Mechanical Engineering， 2005，41（4）：154⁃156.
5	姚康德，王向辉，侯信，等.组织工程相关生物材料［M］.北京：化学工业出版社，2003：100⁃113.
6	徐聪，赵赫，孙岩.生物材料导管修复面神经损伤与再生［J］.中国组织工程研究，2023， 27（7）： 1 089⁃1 095.
	XU C， ZHAO H， SUN Y. Regeneration of facial nerve injury repaired by biomaterial nerve conduits［J］.Chinese Journal of Tissue Engineering Research，2023， 27（7）： 1 089⁃1 095.
7	王晓丹.阿霉素/姜黄素可降解载药体系的制备及药物释放研究［D］. 哈尔滨：哈尔滨工程大学，2018.
8	Jin T R. Mechanisms underlying the metabolic beneficial effect of curcumin intervention：Beyond anti⁃inflammation and anti⁃oxidative stress［J］. Obesity Medicine，2019，13：1⁃5.
9	彭一凡，王增明，王荣荣，等，姜黄素口服纳米晶胶囊的制备及体内外评价［J］.中国药科大学学报， 2021， 52（2）： 211⁃218.
	PENG Y F， WANG Z M， WANG R R， et al. Preparation and in vitro and in vivo evaluation of oral curcumin nanocrystalline capsules［J］. Journal of China Pharmaceutical University， 2021，52（2）：211⁃218.
10	张翠香. 高载药量姜黄素纳米颗粒的制备及体外抗肝癌作用研究［D］. 宜春：宜春学院， 2021.
11	常娜，王巧侠，王小平，等. 姜黄素抗肿瘤机制的研究进展［J］. 生物学杂志，2018，35（1）：97⁃100.
	CHANG N， WANG Q X， WANG X P， et al. The research progress of curcumin antitumor mechanism［J］. Journal of Biology， 2018，35（1）：97⁃100.
12	Mareeswaran P M， Babu E， Sathish V， et al. p⁃Sulfonatocalix［4］arene as carrier for curcumin［J］. New Journal of Chemistry， 2014， 38（3）： 1 336⁃1 345.
13	国家药典委员会.中国药典：2020 年版.四部通则［M］.北京：中国医药科技出版社，2020：268⁃269.
14	李凌冰，谭业帮. 药物从多孔骨架聚合物系统中控制释放的动力学模型［J］.中国生物医学工程学报，2003，22（2）：120⁃126.
	LI L B， TAN Y B. Mathematical model of drug release from porous polymer［J］. Chinese Journal of Biomedical Engineering，2003，22（2）：120⁃126.
15	卿晨. 基于天然蛛丝蛋白制备载药口腔膜剂及其释药性能初探［D］. 大理：大理大学，2022.

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[5]	. Preparation and Properties of Polycaprolactone/Silica Nanocomposites [J]. China Plastics, 2017, 31(06): 65-70 .
[6]	. Study on Thermal Aging Properties of Bamboo Fiber-reinforced Polycaprolactone [J]. China Plastics, 2017, 31(04): 17-23 .
[7]	. Preparation and Properties of Polycaprolactone/Poly(lactic acid)/Bamboo Fiber Composites [J]. China Plastics, 2016, 30(11): 25-31 .
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[9]	. Application of Curcumin as Thermal Stabilizer for PVC [J]. China Plastics, 2015, 29(03): 100-104 .
[10]	LI Dan, CHANG Haibo, MA Conghe, YOU Qianqian, ZHANG Puyu. Synthesis and properties of the polycaprolactone/methyl nano-SiO₂ situ composites [J]. China Plastics, 2013, 27(08): 50-55.
[11]	. Research Progress in Fabrication of Biodegradable Polycaprolactone Foams [J]. China Plastics, 2013, 27(02): 7-13 .
[12]	Zhengliang Wang Xin Deng Suwen Yang Jin Yang. Preparation and Properties of Polycaprolactone/Esterified Starch/Nano-Calcium Carbonate Composites [J]. China Plastics, 2012, 26(05): 40-44 .
[13]	. Research Progress in Foaming Technologies for Biodegradable Polymers [J]. China Plastics, 2012, 26(04): 4-15 .
[14]	MENG Qianni ZHU Guangming XU Shuogui. Biodegradable Shape Memory Polymers and Their Application in Biomedical Engineering [J]. China Plastics, 2011, 25(04): 17-21 .
[15]	CHEN Yadong PENG Zhenbo ZHANG Huibo ZHANG Yongchun. Synthesis and Characterization of Thermally Induced Shape Memory PCL/PU Copolymers [J]. China Plastics, 2010, 24(04): 54-57 .